The way I understand it, when you play subwoofers in stereo they each have their own frequency. Mono they both play one freqency. You should not use two subs on a stereo amp in one chamber. If one channel is weaker than the other you could blow the speaker that is getting less power in a shared, sealed airspace.
I'm actually finding some contradicting information on the subject. There is no performance loss with a partitioned, sealed enclosure. From what I've researched for my new build, I doesn't make much difference since no one plays their subs in stereo and if the subs are out of phaze a partition isn't going to help.
Divided it would marginally be more responsive, acurate and tighter bass. That's the point of doing a sealed enclosure to begin with. Sealed enclosure drivers do eat more power because the cone is alays fighting the air resistance it makes. As a side benifit, sealed enclosures rock in small places.
It's also easier to find problems with each sub.
It adds structural support to the entire enclosure.
I'd partition it.